Elabscience

(KO Validated) PEPCK/PCK2 Polyclonal Antibody

Product Code:
 
E-AB-92385
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
1 / 4
Western blot analysis of extracts from wild type(WT) and PEPCK/PCK2 knockout (KO) 293T cells using PEPCK/PCK2 Polyclonal Antibody at 1:1000 dilution.
2 / 4
Immunohistochemistry of paraffin-embedded rat kidney using PEPCK/PEPCK/PCK2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
3 / 4
Immunofluorescence analysis of NIH-3T3 cells using PEPCK/PEPCK/PCK2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
4 / 4
Immunoprecipitation analysis of extracts of HepG2 cells using PEPCK/PEPCK/PCK2 Polyclonal Antibody. Western blot was performed from the immunoprecipitate u
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Western blot analysis of extracts from wild type(WT) and PEPCK/PCK2 knockout (KO) 293T cells using PEPCK/PCK2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat kidney using PEPCK/PEPCK/PCK2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH-3T3 cells using PEPCK/PEPCK/PCK2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of extracts of HepG2 cells using PEPCK/PEPCK/PCK2 Polyclonal Antibody. Western blot was performed from the immunoprecipitate u

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Further Information

Abbreviation:
PEPCK/PCK2
Background:
This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.
Buffer:
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Calculated MW:
47kDa/70kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200,IP 1:50-1:200
Immunogen:
Recombinant fusion protein of human PEPCK/PCK2
ObservedMW:
71KDa
Purification method:
Affinity purification
Target Synonym:
PCK2; PEPCK; PEPCK-M; PEPCK2; phosphoenolpyruvate carboxykinase 2; mitochondrial
UNIProt ID:
Q16822

Documents