Elabscience

(KO Validated) PARP1 Polyclonal Antibody

Product Code:
 
E-AB-93285
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
1 / 5
Western blot analysis of extracts from wild type(WT) and PARP1 knockout (KO) HeLa cells, using PARP1 Polyclonal Antibody at 1:5000 dilution.
2 / 5
Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
3 / 5
Immunofluorescence analysis of C6 cells using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
4 / 5
Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
5 / 5
Immunofluorescence analysis of U2OS cells using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts from wild type(WT) and PARP1 knockout (KO) HeLa cells, using PARP1 Polyclonal Antibody at 1:5000 dilution.
Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using [KO Validated] PARP1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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E-AB-93285-60uL60uL£224.00
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E-AB-93285-120uL120uL£317.00
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E-AB-93285-200uL200uL£465.00
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Abbreviation:
PARP1
Background:
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
113kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:100,IF 1:50-1:200
Immunogen:
A synthetic peptide of human PARP1
ObservedMW:
114kDa
Purification method:
Affinity purification
Target Synonym:
PARP1; ADPRT; ADPRT 1; ADPRT1; ARTD1; PARP; PARP-1; PPOL; pADPRT-1; poly(ADP-ribose) polymerase 1
UNIProt ID:
P09874

Documents