NSJ Bioreagents

PU.1 Antibody / SPI1

Product Code:
 
NSJ-V3882SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
TFPU1-1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the PU.1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Western blot testing of human THP-1 cell lysate with PU.1 antibody (clone TFPU1-1). Predicted molecular weight ~31 kDa but routinely observed at ~40 kDa.~
2 / 2
IHC staining of FFPE human lymph node with PU.1 antibody (clone TFPU1-1). HIER: bo
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Western blot testing of human THP-1 cell lysate with PU.1 antibody (clone TFPU1-1). Predicted molecular weight ~31 kDa but routinely observed at ~40 kDa.~
IHC staining of FFPE human lymph node with PU.1 antibody (clone TFPU1-1). HIER: bo

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NSJ-V3882SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the PU.1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity). [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 16-170 from the human protein was used as the immunogen for the PU.1 antibody.
Limitation:
This PU.1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P17947

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