NSJ Bioreagents

TNF alpha Antibody

Product Code:
 
NSJ-V2900SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgM kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
P/T2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the TNF-a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC staining of FFPE human pancreas with TNF-a antibody (clone P/T2).~
2 / 2
IHC: Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF-a antibody (clone P/T2).

IHC staining of FFPE human pancreas with TNF-a antibody (clone P/T2).~
IHC: Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF-a antibody (clone P/T2).

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-V2900SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
Application Note:
Optimal dilution of the TNF-a antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

Description:
Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Amino acids 115-130 (NGVELRDNQLVVPSEG) from the human protein were used as the immunogen for the TNF-a antibody.
Limitation:
This TNF-a antibody is available for research use only.
Localization:
Cytoplasmic and extracellular (secreted)
Purity:
PEG precipitation
Uniprot #:
P01375

Documents