NSJ Bioreagents

NKX2.1 Antibody / TTF-1

Product Code:
 
NSJ-V2894SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
NX2.1/690
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the TTF-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC: Formalin-fixed, paraffin-embedded human lung Adenocarcinoma stained with TTF-1 antibody (NX2.1/690)~
2 / 2
IHC: Formalin-fixed, paraffin-embedded human thyroid stained with TTF-1 antibody (NX2.1/690)

IHC: Formalin-fixed, paraffin-embedded human lung Adenocarcinoma stained with TTF-1 antibody (NX2.1/690)~
IHC: Formalin-fixed, paraffin-embedded human thyroid stained with TTF-1 antibody (NX2.1/690)

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CodeSizePrice
NSJ-V2894SAF-100UG100 ug£534.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Optimal dilution of the TTF-1 antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1, also called NKX2.1. TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant protein was used as the immunogen for the TTF-1 antibody.
Limitation:
This TTF-1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P43699

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