Neurofilament Antibody (-Heavy) / NF-H / NEFH

Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

The stated application concentrations are suggested starting points. Titration of the recombinant NF-H antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H).

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Recombinant human protein (heavy subunit) was used as the immunogen for the recombinant NF-H antibody.

This recombinant NF-H antibody is available for research use only.

Cytoplasmic, membranous

Protein A affinity

P12036