NSJ Bioreagents

CD15 Antibody / SSEA-1

Product Code:
 
NSJ-V7257SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
FUT4/1478R
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant CD15 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Immunofluorescent staining of human U937 cells with recombinant CD15 antibody (clone FUT4/1478R, green) and Reddot nuclear stain (red).~
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IHC testing of FFPE human Hodgkin's lymphoma and recombinant CD15 antibody (clone FUT4/1478R). Requi
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Immunofluorescent staining of human U937 cells with recombinant CD15 antibody (clone FUT4/1478R, green) and Reddot nuclear stain (red).~
IHC testing of FFPE human Hodgkin's lymphoma and recombinant CD15 antibody (clone FUT4/1478R). Requi

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NSJ-V7257SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant CD15 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Purified human neutrophils were used as the immunogen for the recombinant CD15 antibody.
Limitation:
This recombinant CD15 antibody is available for research use only.
Localization:
Cytoplasmic and cell surface
Purity:
Protein A affinity
Uniprot #:
P22083

Documents