Pan Cytokeratin Antibody Cocktail (Acidic + Basic)

Flow cytometry: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood,Immunofluorescence: 1:50-1:100

Optimal dilution of the Pan Cytokeratin antibody should be determined by the researcher.

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

FITC Conjugate

500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Human epidermal keratin was used as the immunogen for this Pan Cytokeratin antibody.

This Pan Cytokeratin antibody is available for research use only.

Cytoplasmic

Protein G affinity chromatography