NSJ Bioreagents

Estrogen Receptor beta 2 Antibody

Product Code:
 
NSJ-V8668SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
57/3
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Estrogen Receptor beta 2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Flow cytometry testing of PFA-fixed human MCF7 cells with Estrogen Receptor beta 2 antibody (clone 57/3); Red=isotype control, Blue= Estrogen Receptor beta 2 antibody.
2 / 3
IHC staining of FFPE human testis with Estrogen Receptor beta 2 antibody (clone 57/3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free Estrogen Receptor beta 2 antibody (clone 57/3) as confirmation of integrity and purity.
❮❯

Flow cytometry testing of PFA-fixed human MCF7 cells with Estrogen Receptor beta 2 antibody (clone 57/3); Red=isotype control, Blue= Estrogen Receptor beta 2 antibody.
IHC staining of FFPE human testis with Estrogen Receptor beta 2 antibody (clone 57/3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Estrogen Receptor beta 2 antibody (clone 57/3) as confirmation of integrity and purity.

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NSJ-V8668SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 2-4ug/million cells,Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the Estrogen Receptor beta 2 antibody should be determined by the researcher.
Description:
This MAb recognizes the estrogen receptor beta 2 isoform, expressed in cell nuclei of a range of human tissues including ovary, placenta, testis and vas deferens. No cross-reactivity is seen with estrogen receptor beta 1 isoform. This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Amino acids CGMKMETLLPEATMEQ from the C terminus of the human estrogen receptor beta 2 isomer were used as the immunogen for the Estrogen Receptor beta 2 antibody.
Limitation:
This Estrogen Receptor beta 2 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
Q92731

Documents