ELK Biotechnology

Cattle MMP2 (Matrix Metalloproteinase 2) ELISA Kit

Product Code:
 
ELK0383
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Host Type:
 
Bovine (Cattle)
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK0383-48T48T£359.00
Quantity:
ELK0383-96T96T£477.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Alternative Names:
CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
156.25-10000 pg/mL
Sample Type:
serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
32.2 pg/mL
Standard:
10000 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle MMP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle MMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle MMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Cattle MMP2 in the samples is then determined by comparing the OD of the samples to the standard curve.