ELK Biotechnology

Horse 5-HT (5-Hydroxytryptamine) ELISA Kit

Product Code:
 
ELK0384
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Host Type:
 
Equine (Horse)
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK0384-48T48T£261.00
Quantity:
ELK0384-96T96T£335.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
5HT; Serotonin; N-Methyl-Gamma
Assay Length:
2.5h
Assay Type:
Competitive Inhibition
Detection Range:
14.07-900 ng/mL
Sample Type:
serum, plasma, tissue homogenates
Sensitivity:
6.12 ng/mL
Standard:
900 ng/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Horse 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.