ELK Biotechnology

Mouse OVA sIgG (Ovalbumin specific Immunoglobulin G) ELISA Kit

Product Code:
 
ELK0420
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Host Type:
 
Mouse
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK0420-48T48T£261.00
Quantity:
ELK0420-96T96T£335.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
OVA sIg; Ovalbumin specific Immunoglobulin G
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
3.13-200 ng/mL
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
1.12 ng/mL
Standard:
200 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse OVA sIgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OVA sIgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse OVA sIgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse OVA sIgG in the samples is then determined by comparing the OD of the samples to the standard curve.