NSJ Bioreagents

TOX Antibody (Antigen affinity purified)

Product Code:
 
NSJ-RQ7024
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the TOX antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE mouse lymph tissue with TOX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human intestinal cancer tissue with TOX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human 1) Jurkat, 2) Ramos and 3) HL60 cell lysate with TOX antibody. Predicted molecular weight ~57 kDa.
4 / 4
Flow cytometry testing of human HL60 cells with TOX antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TOX antibody.
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IHC staining of FFPE mouse lymph tissue with TOX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer tissue with TOX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) Jurkat, 2) Ramos and 3) HL60 cell lysate with TOX antibody. Predicted molecular weight ~57 kDa.
Flow cytometry testing of human HL60 cells with TOX antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TOX antibody.

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CodeSizePrice
NSJ-RQ7024-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TOX antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Thymocyte selection-associated high mobility group box protein TOX is a protein that in humans is encoded by the TOX gene. The protein encoded by this gene contains a HMG box DNA binding domain. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. This protein may function to regulate T-cell development.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-S495) was used as the immunogen for the TOX antibody.
Limitation:
This TOX antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
O94900

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