NSJ Bioreagents

ALDH3A1 Antibody (Antigen affinity purified)

Product Code:
 
NSJ-RQ7034
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Aldehyde dehydrogenase 3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A549 cells with Aldehyde dehydrogenase 3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
IHC staining of FFPE human liver cancer tissue with Aldehyde dehydrogenase 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 3
Western blot testing of human A549 cell lysate with Aldehyde dehydrogenase 3 antibody. Predicted molecular weight: ~51 kDa.
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Immunofluorescent staining of FFPE human A549 cells with Aldehyde dehydrogenase 3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human liver cancer tissue with Aldehyde dehydrogenase 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human A549 cell lysate with Aldehyde dehydrogenase 3 antibody. Predicted molecular weight: ~51 kDa.

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NSJ-RQ7034-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Aldehyde dehydrogenase 3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
ALDH3A1 (Aldehyde Dehydrogenase, Family 3, Subfamily A, Member 1), also known as ALDH3, is an enzyme that in humans is encoded by the ALDH3A1 gene. By in situ hybridization, Hiraoka et al.(1995) mapped the ALDH3 gene to 17p11.2. ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Y413-H453) was used as the immunogen for the Aldehyde dehydrogenase 3 antibody.
Limitation:
This Aldehyde dehydrogenase 3 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P30838

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