NSJ Bioreagents

UBE2O Antibody / Ubiquitin-conjugating enzyme E2 O

Product Code:
 
NSJ-RQ7542
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the UBE2O antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human Daudi cells with UBE2O antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBE2O antibody.~
2 / 2
Western blot testing of 1) human 293T, 2) human HeLa, 3) human Jurkat and 4
❮❯

Flow cytometry testing of human Daudi cells with UBE2O antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBE2O antibody.~
Western blot testing of 1) human 293T, 2) human HeLa, 3) human Jurkat and 4

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CodeSizePrice
NSJ-RQ7542-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the UBE2O antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ubiquitin-conjugating enzyme E2 O is a protein that in humans is encoded by the UBE2O gene. Enables ubiquitin conjugating enzyme activity and ubiquitin protein ligase activity. Involved in positive regulation of BMP signaling pathway; protein ubiquitination; and retrograde transport, endosome to Golgi. Located in cytoplasm and nuclear body.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R150-A1283) was used as the immunogen for the UBE2O antibody.
Limitation:
This UBE2O antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q9C0C9

Documents