NSJ Bioreagents

CLASP1 Antibody / CLIP-associating protein 1

Product Code:
 
NSJ-RQ7358
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the CLASP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 8
Immunofluorescent staining of FFPE human colorectal adenocarcinoma tissue with CLASP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 8
Immunofluorescent staining of FFPE rat brain tissue with CLASP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 8
IHC staining of FFPE human prostate cancer tissue with CLASP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human esophageal squamous carcinoma tissue with CLASP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE human colorectal adenocarcinoma tissue with CLASP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE squamous cell carcinoma of human cervix tissue with CLASP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 8
IHC staining of FFPE rat brain tissue with CLASP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 8
Western blot testing of 1) human U-87 MG, 2) human HeLa, 3) human HEL, 4) human K562, 5) rat brain and 6) rat C6 cell lysate with CLASP1 antibody. Expected molecular weight: 162-169 kDa (multiple isoforms).
❮❯

Immunofluorescent staining of FFPE human colorectal adenocarcinoma tissue with CLASP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE rat brain tissue with CLASP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human prostate cancer tissue with CLASP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with CLASP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with CLASP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE squamous cell carcinoma of human cervix tissue with CLASP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with CLASP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human U-87 MG, 2) human HeLa, 3) human HEL, 4) human K562, 5) rat brain and 6) rat C6 cell lysate with CLASP1 antibody. Expected molecular weight: 162-169 kDa (multiple isoforms).

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CodeSizePrice
NSJ-RQ7358-100UG100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CLASP1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Cytoplasmic linker associated protein 1, also known as CLIP-associating protein 1 and CLASP1, is a protein which in humans is encoded by the CLASP1 gene. CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-D243) was used as the immunogen for the CLASP1 antibody.
Limitation:
This CLASP1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q7Z460

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