H9-derived Cardiomyocytes

H9-CM are cryopreserved at passage one after enrichment and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. Includes one vial of H9-CM, 50mL of Cat# 5921 Cardiomyocyte Plating Medium-basal, and 1 mL of Cat# 5972 Cardiomyocyte Plating Medium Supplement (50X).

Human primary cardiomyocytes and cardiac tissue are superior modeling systems for heart disease studies, drug discovery and toxicity testing compared to animal cells or immortalized cell lines. Human adult primary cardiomyocytes are difficult to obtain due to limited availability of cardiac tissue and they do not typically proliferate in culture. Cardiomyocytes derived from human pluripotent stem cells (HPSC) can potentially provide an unlimited supply of cardiomyocytes for drug screening, toxicity testing and developmental studies. HPSC-derived Cardiomyocytes (H9-CM) from ScienCell Research Laboratories are differentiated from a pluripotent stem cell line (H9) and enriched for contracting cells. Through the temporal modulation of canonical Wnt signaling and growth factor induction, monolayer-cultured HPSCs are differentiated to contracting cardiomyocytes under serum-free and feeder-free conditions at high efficiency. H9-CM are cryopreserved at passage one after enrichment and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. Cells are characterized by immunofluorescence with antibodies specific to α-Actinin and Sarcomeric Tropomyosin. H9-CM are negative for mycoplasma, bacteria, yeast and fungi. H9-CM are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories. H9-CM are not recommended for expanding since the cells show limited proliferation capability in culture. Recommended Medium - It is recommended to use Cardiac Myocyte Medium (CMM, Cat. #6201) for the culturing of H9-CM in vitro .