Anti-Human/Mouse/Rat Glycophorin A - Purified
Antibody Isotype:
Mouse IgG1 κ
Antibody Clonality:
Monoclonal
Applications:- Functional Study
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
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This product comes from:
US.
Typical lead time:
14-21 working days.
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Further Information
Glycophorin A (CD235a) is expressed on hematopoietic stem cells,
erythroid cells, and erythrocytes. Erythroleukemia can express glycophorin A on neoplastic
erythroblasts.
0.5 mg/ml
Purified No Carrier Protein
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Membrane preparation from splenic hairy cell leukemia
Glycophorins are transmembrane proteins of red blood cells (RBC)1 . There are four glycophorin encoding genes (A, B, C, and D), and a fifth pseudogene (E), which has no protein product.
Glycophorin A, B, and E are tandemly arranged repeats on chromosome 4 and are prone to
rearrangements that lead to rare blood groups. Glycophorin A and B carry MN and Ss blood group antigens.
Glycophorin A is a type I glycoprotein carrying sialylated O- and N-glycans, and most of the O-glycans are NeuAcα2-3Galβ1-3(NeuAcα2-6)GaINAc-tetrasaccharide chains linked to serine or
threonine residues 2 . The abundance of glycophorin A on the surface of RBCs is thought to create
a negatively charged cell surface of complex glycans that allows for RBC circulation without
sticking to other cells or blood vessel walls1 .
Glycophorin A binds CD170 3 (Siglec 5, which mediates sialic-acid dependent binding to cells4),
influenza virus, and EBA-175, the erythrocyte binding antigen of Plasmodium falciparum, the
parasite responsible for malaria in Africa 2 . Babesia divergens, which like Plasmodium is a
parasite in the Apicomplexa phylum, also uses glycophorins A and B to enter RBCs. Other
pathogens that bind to glycophorin A on the surface of RBCs include: some strains of
Escherichia coli, reoviruses (a group of viruses that includes rotavirus), encephalomyocarditis virus, and hepatitis A 2 . Glycophorin A also interacts with influenza virus but because influenza virus cannot replicate in anucleated RBCs glycophorin A might be acting as a decoy receptor to divert infection away from other tissues.
JC159 was generated using splenic hairy cell leukemia cells as immunogen and reacts with a
formalin-fixed, paraffin-embedded resistant epitope, likely between amino acids 27 and 40, on
the extracellular domain5.
2993
Glycophorin A