NSJ Bioreagents

RTCB Antibody / C22orf28

Product Code:
 
NSJ-RQ7879
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RTCB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with RTCB antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HeLa, 2) Jurkat, 3) HL60 and 4) 293T cell lysate with RTCB antibody. Predicted molecular weight ~55 kDa.
3 / 3
Flow cytometry testing of human HepG2 cells with RTCB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RTCB antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with RTCB antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) Jurkat, 3) HL60 and 4) 293T cell lysate with RTCB antibody. Predicted molecular weight ~55 kDa.
Flow cytometry testing of human HepG2 cells with RTCB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RTCB antibody.

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CodeSizePrice
NSJ-RQ7879-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
RNA 2',3'-cyclic phosphate and 5'-OH ligase is a protein that in humans is encoded by the RTCB gene. Enables RNA ligase (ATP) activity and vinculin binding activity. Involved in tRNA splicing, via endonucleolytic cleavage and ligation. Located in cytosol and nucleoplasm. Part of tRNA-splicing ligase complex.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids H227-G505) was used as the immunogen for the RTCB antibody.
Localisation:
Nuclear, cytoplasmic
PM_Meta_Key:
Product_Images
PM_Meta_Value:
http://www.nsjbio.com/get_image.php?catalog_no=RQ7879&type=image1~IHC staining of FFPE human lung cancer tissue with RTCB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.|http://www.nsjbio.com/get_image.php?catalog_no=RQ7879&type=image2~IHC staining of FFPE human parotid acinar cell carcinoma tissue with RTCB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.|http://www.nsjbio.com/get_image.php?catalog_no=RQ7879&type=image3~IHC staining of FFPE human ovarian cancer tissue with RTCB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.|http://www.nsjbio.com/get_image.php?catalog_no=RQ7879&type=image4~IHC staining of FFPE human breast cancer tissue with RTCB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.|http://www.nsjbio.com/get_image.php?catalog_no=RQ7879&type=image5~IHC staining of FFPE human skin squamous cell carcinoma tissue with RTCB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.|http://www.nsjbio.com/get_image.php?catalog_no=RQ7879&type=image6~IHC staining of FFPE mouse brain tissue with RTCB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.|http://www.nsjbio.com/get_image.php?catalog_no=RQ7879&type=image7~IHC staining of FFPE rat brain tissue with RTCB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.|http://www.nsjbio.com/get_image.php?catalog_no=RQ7879&type=image8~Immunofluorescent staining of FFPE human HeLa cells with RTCB antibody (red). HIER: steam section in pH6 citrate buffer for 20 min.|http://www.nsjbio.com/get_image.php?catalog_no=RQ7879&type=image9~Western blot testing of human 1) HeLa, 2) Jurkat, 3) HL60 and 4) 293T cell lysate with RTCB antibody. Predicted molecular weight ~55 kDa.|http://www.nsjbio.com/get_image.php?catalog_no=RQ7879&type=image10~Western blot testing of human 1) HeLa, 2) Jurkat, 3) 293T and 4) HepG2 cell lysate with RTCB antibody. Predicted molecular weight ~55 kDa.|http://www.nsjbio.com/get_image.php?catalog_no=RQ7879&type=image11~Flow cytometry testing of fixed and permeabilized human HeLa cells with RTCB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RTCB antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q9Y3I0