NSJ Bioreagents

ATF2 Antibody

Product Code:
 
NSJ-V9624
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-ATF2-1B4
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the ATF2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Immunofluorescent staining of human HeLa cells stained using ATF2 antibody (green, clone PCRP-ATF2-1B4) and phalloidin (red).
2 / 5
FACS staining of PFA-fixed human HeLa cells with ATF2 antibody (blue, clone PCRP-ATF2-1B4), and unstained cells (red).
3 / 5
FACS staining of PFA-fixed human U-87 MG cells with ATF2 antibody (blue, clone PCRP-ATF2-1B4), and unstained cells (red).
4 / 5
SDS-PAGE analysis of purified, BSA-free ATF2 antibody (clone PCRP-ATF2-1B4) as confirmation of integrity and purity.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ATF2 antibody (clone PCRP-ATF2-1B4). These results demonstrate the foremost specificity of the PCRP-ATF2-1B4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of human HeLa cells stained using ATF2 antibody (green, clone PCRP-ATF2-1B4) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with ATF2 antibody (blue, clone PCRP-ATF2-1B4), and unstained cells (red).
FACS staining of PFA-fixed human U-87 MG cells with ATF2 antibody (blue, clone PCRP-ATF2-1B4), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free ATF2 antibody (clone PCRP-ATF2-1B4) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ATF2 antibody (clone PCRP-ATF2-1B4). These results demonstrate the foremost specificity of the PCRP-ATF2-1B4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9624-20UG20 ug£264.00
Quantity:
NSJ-V9624-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Description:
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cells DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human ATF2 protein was used as the immunogen for the ATF2 antibody.
Localisation:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
P15336