Elabscience

Cell Mitochondrial Complex II Activity Assay Kit

Product Code:
 
E-BC-K835-M
Product Group:
 
Cell Based Assays
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Activity Assay
Shipping:
 
Ice bag
Storage:
 
This product can be stored at -20°C for 12 months with shading light.

 

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CodeSizePrice
E-BC-K835-M-48T48T£283.00
Quantity:
E-BC-K835-M-96T96T£386.00
Quantity:
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Further Information

Assay Time:
30 min
Detection Instrument:
Microplate reader
Detection Range:
0.54-17.66 U/L
Detection Type:
Colorimetric method
Inter cv:
3.0-6.0%
Intra cv:
2.0-5.0%
Result Type:
Enzyme Activity
Sensitivity:
0.54 U/L
Target Synonym:
succinate-coenzyme Q reductase
Test Principle:
Mitochondrial complex II, also known as succinate-coenzyme Q reductase, is widely found in the mitochondria of animals, plants, microorganisms and cultured cells. It catalyzes the oxidation of succinic acid in the TCA cycle to fumaric acid, which converts the ubiquinone to a reduced form through the basic unit structures of the complex such as iron-sulfur proteins. Coenzyme Q, the catalytic product of mitochondrial complex II, can further reduce 2, 6-dichloroindoxol, which has a characteristic absorption peak at 600 nm. Therefore, the activity of mitochondrial complex II can be quantified by measure the change OD value at 600 nm.
Tested Sample Types:
cell

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