ProSci

Brain Membrane Lysate

Product Code:
 
PSI-21-358
Supplier:
 
ProSci
Host Type:
 
Mouse
Regulatory Status:
 
RUO
Application:
 
Western Blot (WB)
Shipping:
 
Dry Ice
Storage:
 
Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
 

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CodeSizePrice
PSI-21-358-0.1mg0.1mg£599.00
Quantity:
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This product comes from: United States.
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Further Information

Application Note:
Brain membrane lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
Background:
Mouse brain tissue membrane protein lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The mouse brain tissue membrane is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the brain tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated brain tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Buffer:
HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
Concentration:
2 mg/mL or better
Diagnosis:
Normal
Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. Also, for further information on the biosafety classification of human etiologic agents, please consult the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention's Office of Health and Safety (www.cdc.gov/od/ohs).
Physical State:
Liquid
Type:
Mouse brain tissue

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