Ready-To-Use 3D Human Mesenchymal Stem Cell Spheroids - 48 plates

Mesenchymal stem cells (MSCs) have emerged as a leading candidate for use in cell therapy due to its multifunctional properties. Their ability to differentiate into specific cell types their immune-suppressive properties as well as their potent tropic effects during tissue repair make them invaluable in cell therapy [1]. Several lines of evidence has shown that culturing MSC as three-dimensional (3D) spheroids enhances a wide range of biological properties [1-3]. Traditionally the generation of 3D MSC spheroids can be tedious and rate limiting. In order to facilitate high-throughput MSC studies ScienCell offers ready-to-use 3D mesenchymal stem cell spheroids (SP3D-HMSCS) which can be thawed and plated directly in multiwell plates. By RT-qPCR analysis we demonstrate that SP3D-HMSCS have elevated mRNA expression of pluripotency markers such as Sox2 Nanog and CD90 indicating the enhanced stemness. Furthermore chemotaxic factor (CXCR4) anti-inflammatory molecule (PEG2) as well as other endogenous growth factors (VEGF and FGF2) are highly expressed in 3D MSC spheroids thereby promoting the migration colonization and viability of MSC. SP3D-HMSCS are therefore a great model for examining the functions of mesenchymal stem cells in cell therapy and tissue regeneration.

ScienCell's ready-to-use 3D mesenchymal stem cell spheroids (SP3D-HMSCS) can be thawed and plated directly in multiwell plates. By RT-qPCR analysis we demonstrate that SP3D-HMSCS have elevated mRNA expression of pluripotency markers such as Sox2 Nanog and CD90. Furthermore chemotaxic factor (CXCR4) anti-inflammatory molecule (PEG2) as well as other endogenous growth factors (VEGF and FGF2) are highly expressed in 3D MSC spheroids.