ELK Biotechnology

Rat CRP(C Reactive Protein) ELISA Kit

Product Code:
 
ELK9996
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Host Type:
 
Rat
Regulatory Status:
 
RUO

 

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CodeSizePrice
ELK9996-48T48T£232.00
Quantity:
ELK9996-96T96T£303.00
Quantity:
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Further Information

Alternative Names:
C-RP; PTX1; Pentraxin-Related; Pentraxin 1
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.78-50 ug/mL
Sample type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.29 ug/mL
Standard:
50 ug/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rat CRP in the samples is then determined by comparing the OD of the samples to the standard curve.

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