Chimeric HLA-A*02:01 (ma3) &B2M&WT-1 (RMFPNAPYL) Monomer Protein (MHC-HM414)
Product Code:
KAC-MHC-HM414
Shipping:
Shipped at ambient temperature.
Storage:
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Immobilized Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of ?the MHC Class I, B2M and RMFPNAPYL peptide of ?the WT-1.
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Recombinant Chimeric HLA-A*02:01(mα3)&B2M&WT-1 (RMFPNAPYL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide.
Less than 1EU per ug by the LAL method.
Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(B2M) and RMFPNAPYL peptide
HEK293
Lyophilized
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&P61769(B2M)&RMFPNAPYL
The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33
C-His-Avi
Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer
