KactusBio Inc.

Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein (MHC-HM431B)

Product Code:
 
KAC-MHC-HM431B
Product Group:
 
MHC Monomers
Supplier:
 
KactusBio Inc.
Host Type:
 
Human
Regulatory Status:
 
RUO
Shipping:
 
Shipped at ambient temperature.
Storage:
 
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Immobilized Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA
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Immobilized Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA 

Immobilized Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA
Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%
The purity of Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer was greater than 95% as determined by SEC-HPLC.

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CodeSizePrice
KAC-MHC-HM431B-100UG100UG£313.00
Quantity:
KAC-MHC-HM431B-500UG500UG£1,016.00
Quantity:
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This product comes from: United States.
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Further Information

Background:
The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of ?the MHC Class I, B2M and RMFPNAPYL peptide of ?the WT-1.
Biotinylated:
yes
Description:
Recombinant Biotinylated Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide.
Endotoxin:
Less than 1EU per ug by the LAL method.
Exact Sequence:
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide
Expression System:
HEK293
Form:
Lyophilized
Formulation:
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Gene/Protein ID:
A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL
Molecular Weight:
The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
Purity:
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Reconstitution:
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Synonyms:
MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33
Tag:
C-His-Avi
Target:
HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer

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