KactusBio Inc.

Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein (MHC-HM402B)

Product Code:
 
KAC-MHC-HM402B
Product Group:
 
MHC Monomers
Supplier:
 
KactusBio Inc.
Host Type:
 
Human
Regulatory Status:
 
RUO
Shipping:
 
Shipped at ambient temperature.
Storage:
 
-20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test)
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Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test) 

Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test)
Immobilized Tebentafusp, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag with the EC50 of 11.8ng/ml determined by ELISA
Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%
The purity of Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer was greater than 95% as determined by SEC-HPLC.

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CodeSizePrice
KAC-MHC-HM402B-100UG100UG£313.00
Quantity:
KAC-MHC-HM402B-500UG500UG£1,016.00
Quantity:
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This product comes from: United States.
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Further Information

Background:
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of ?the MHC Class I and YLEPGPVTA peptide of ?the gp100.
Biotinylated:
yes
Description:
Recombinant Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide.
Endotoxin:
Less than 1EU per ug by the LAL method.
Exact Sequence:
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide
Expression System:
HEK293
Form:
Lyophilized
Formulation:
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Gene/Protein ID:
A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA
Molecular Weight:
The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.
Purity:
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Reconstitution:
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Synonyms:
HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100
Tag:
C-His-Avi
Target:
HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer

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