Enhanced Primary Human Liver Sinusoidal Endothelial Cells

Enhanced Primary Human Liver Sinusoidal Endothelial Cells are expanded primary cells that retain the properties of primary liver sinusoidal endothelial cells allowing for a reliable in vitro research model. These cells are ready-to-use for applications in research and development, such as viral infections, screening, co-cultures, transient transfection, and for toxicity studies. The cells offer a unique ability to be kept in longer term culture when compared to standard primary liver sinusoidal endothelial cells which does not proliferate well, in vitro.The cells are ready-to-use after thawing. abm recommends passaging cells 1-2 times; beyond this limit cells may exhibit phenotypic changes and express senescence genes.

PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Human Liver Sinusoidal Endothelial Cell Thawing Medium (TM111) + Human Liver Sinusoidal Endothelial Cell Media Kit (TM112), 37.0°C, 5% CO2.Note: TM111 is required for optimal recovery of cells post-thaw. Cells are grown using TM112. 

Adherent, endothelial-like

MMR+, CD31+

10,000

Liver