Thermolabile UDG

Thermolabile UDG is a uracil-DNA glycosylase which catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA. The release of uracil results in high susceptibility to hydrolytic cleavage at elevated temperatures or high pH. This function allows for many applications such as the prevention of carryover contamination during PCR or qPCR. By incorporating uracil in the reactions, the degradation of previous reaction products can be ensured and any contamination can be prevented. Furthermore, this enzyme is sensitive to heat and can be rapidly and completely inactivated at temperatures above 50°C which also prevents any downstream interference.