TO1-3PEG-Biotin Fluorophore

RNA Mango technology is based on the specific binding of the RNA Mango Aptamer and a Thizole Orange (TO) bi-functional dye. The main features of this technology is the tight binding between the dye and aptamer (KD ~ 3nM) , and the strong ~1000X enhancement of the dye?s fluorescence when bound to the Mango apatmer (Fluorescent enhancement FE=1,100).The TO dye has a number of other desirable properties including: -small size -lack of toxicity -plasma and nuclear membrane permeability -short intracellular half-life -the accessibility of a broad wavelength range simply via substitutions and alterations to the TO structure TO1-biotin is the standard variety of TO dye for RNA Mango experiments; other dye variants are available. Learn more about the RNA Mango technology here.