YO3-3PEG-Biotin Fluorophore

YO3-3PEG-Biotin is a small bifunctional fluorophore that has low unbound fluorescence. When bound to Mango aptamers, it exhibits peak excitation maxima of 580 nm (with additional excitation at 260 nm) and peak fluorescence emission of 620 nm. Mango aptamers enhance the fluorescence of YO3-3PEG-Biotin (binding requires KCl, 61-fold brighter with Mango III A10U), emitting in the orange region of the visible spectrum (unpublished data from Unrau Laboratory). YO3-3PEG-Biotin may serve as a FRET acceptor, when paired with GFP-emitting fluorophores1. RNA Mango technology is based on the specific binding of the RNA Mango Aptamer and a Thizole Orange (TO) bi-functional dye. The TO dye has a number of other desirable properties including: -Small size -Lack of toxicity -Plasma and nuclear membrane permeability -Short intracellular half-life -The accessibility of a broad wavelength range simply via substitutions and alterations to the TO structure TO3-3PEG-Biotin has binding affinities to Mango I, Mango II, Mango III, and Mango IV aptamers. Learn more about the RNA Mango technology here.