Chemodex

4-Methylumbelliferyl-beta-D-glucopyranoside

Product Code:
 
CDX-M0099
Product Group:
 
Dyes, Stains, and Probes
Supplier:
 
Chemodex
Regulatory Status:
 
RUO
Shipping:
 
Ambient
Storage:
 
Short term: +4°C. Long term: -20°C
Chemical Structure

Chemical Structure 

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CDX-M0099-G0011 g£110.00
Quantity:
CDX-M0099-G0055 g£417.00
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This product comes from: Switzerland.
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  • Further Information
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Further Information

Appearance:
White to off-white powder.
CAS:
18997-57-4
Description:
4-Methylumbelliferyl-beta-D-glucopyranoside (MUD) is a non-fluorescent but sensitive fluorogenic substrate for beta-glucosidase and beta-glucocerebrosidase (also known as glucosylceramidase) yielding a blue fluorescent solution. It is a fluorogenic MU substrate for detecting glucosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. It is employed in the detection of beta-glucosidase as an indicator of Enterococci. It has been extensively used in work on Gaucher's disease, a lipid storage disease characterized by the accumulation of glucocerebroside due to a genetic deficiency of a beta-glucosidase. The use of 4-Methylumbelliferyl-beta-D-glucopyranoside has also been reported in a rapid method for identifying bacterial enzymes. Hydrolysis of MUD releases the fluorescent product 4-MU. Spectral Data. lambdaEx/lambdaEm= 360/450 nm (cleaved product, pH-dependent).
EClass:
32160000
Form:
solid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13-,14+,15-,16-/m1/s1
InChi Key:
YUDPTGPSBJVHCN-YMILTQATSA-N
MDL:
MFCD00063694
Molecular Formula:
C16H18O8
Molecular Weight:
338.31
Package Type:
Vial
Purity:
>97% (NMR)
SMILES:
O[C@H]1[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@H](CO)[C@@H](O)[C@@H]1O
Solubility:
Soluble in DMSO or DMF.
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.

Documents

References

(1) S.P. Peters, et al.; Clin. Chim. Acta 60, 391 (1975) | (2) D.M. Broadhead & J. Butterworth; Clin. Chim. Acta 75, 155 (1977) | (3) A. Basu & R.H. Glew; J. Biol. Chem. 260, 13067 (1985) | (4) K.J. Panosian & S.C. Edberg; J. Clin. Microbiol. 27, 1719 (1989) | (5) S. Nakagawa, et al.; Clin. Chim. Acta. 118, 99 (1992) | (6) B. Setlow, et al.; J. Appl. Microbiol. 96, 1245 (2004) | (7) R. Kwapiszewski, et al.; Biomed. Microdevices 13, 431 (2011) | (8) M. Shanmuganathan & P. Britz-McKibbin; Anal. Bioanal. Chem. 399, 2843 (2011) | (9) L. Oftedal, et al.; Sci. Rep. 10, 22098 (2020)