Abnova Corporation

SMAD3 (phospho S423/425) polyclonal antibody

Product Code:
 
PAB11304
Product Group:
 
Primary Antibodies
Supplier:
 
Abnova Corporation
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
 

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CodeSizePrice
PAB11304-100ug100 ug£709.00
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Further Information

Antigen Target Species:
Human
Form:
Liquid
Gene Alias:
DKFZp586N0721|DKFZp686J10186|HSPC193|HsT17436|JV15-2|MADH3|MGC60396
Gene Description:
SMAD family member 3
Gene ID:
4088
Gene Name:
SMAD3
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding S423/S425 of human SMAD3.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product Description:
Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD3.
Protein Accession:
NP_005893;P84022
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Recommend Dilutions:
ELISA (1:75000-1:300000)
Western Blot (1:2000-1:20000)
Immunohistochemistry (1:500)
The optimal working dilution should be determined by the end user.
Specificity:
Reactivity occurs against human Smad3 pS423 and pS425 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members.
Storage Buffer:
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)

References

  • http://www.ijcep.com/files/ijcep0006530.pdf
  • http://www.ncbi.nlm.nih.gov/pubmed/18922473
  • http://www.ncbi.nlm.nih.gov/pubmed/12809600
  • http://www.ncbi.nlm.nih.gov/pubmed/14534577