Further Information
TGFB4; EBAF; LEFTA; LEFTYA; Endometrial Bleeding Associated Factor; Left-right determination factor A; Transforming growth factor beta-4
3h
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
15.62-1000pg/mL
48T, 96T, 96T?5, 96T?10, 96T?100
Left/Right Determination Factor 2
Double-antibody Sandwich
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Left/Right Determination Factor 2 (LEFTY2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Left/Right Determination Factor 2 (LEFTY2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Neuro science;
Serum, plasma and other biological fluids.
The minimum detectable dose of this kit is typically less than 5.5pg/mL
This assay has high sensitivity and excellent specificity for detection of Left/Right Determination Factor 2 (LEFTY2).
No significant cross-reactivity or interference between Left/Right Determination Factor 2 (LEFTY2) and analogues was observed.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 2 (LEFTY2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 2 (LEFTY2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Left/Right Determination Factor 2 (LEFTY2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Left/Right Determination Factor 2 (LEFTY2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
P57785
