AdipoGen Life Sciences

Nampt (Visfatin/PBEF) (mouse/rat) Dual ELISA Kit

Product Code:
 
AG-45A-0007Y
Product Group:
 
ELISA Kits
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
Blue Ice
Storage:
 
4°C
1 / 1
Standard curve
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Standard curve

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AG-45A-0007YEK-KI0196 wells£600.00
Quantity:
AG-45A-0007YTP-KI012 x 96 wells£1,010.00
Quantity:
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Further Information

Alternate Names/Synonyms:
Nicotinamide Phosphoribosyltransferase; PBEF1; Pre-B Cell Colony Enhancing Factor 1
Assay Type:
Sandwich
Detection Type:
Colorimetric
EClass:
32160000
Form (Short):
liquid
Handling Advice:
After standard reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Plate and reagents should reach room temperature before use.
Long Description:
ELISA Assay. Detects mouse and rat Nampt. Does not cross-react with human Nampt, mouse adiponectin, mouse resistin, mouse vaspin or mouse RBP4. Colorimetric assay. Sample Types: Serum. Range: 0.5 to 32ng/ml. Sensitivity: 50pg/ml. Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDA adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. It has been shown that Nampt expression is increased in different diseases, like diabetes, obesity, fetal growth retardation, sepsis, inflammatory bowel disease, Crohn?s disease and rheumatoid arthritis. Furthermore, Nampt might be a marker of pre-eclampsia and of endothelial health.
NCBI, Uniprot Number:
Q99KQ4
Package Type:
Box
Product Description:
Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDA adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. It has been shown that Nampt expression is increased in different diseases, like diabetes, obesity, fetal growth retardation, sepsis, inflammatory bowel disease, Crohn?s disease and rheumatoid arthritis. Furthermore, Nampt might be a marker of pre-eclampsia and of endothelial health. Recently NAMPT extracellular (eNAMPT) has been shown to be secreted from macrophages in muscles and to triggers a proliferative signal of muscle stem cell (MusC) by binding to CCR5 located at the surface receptor of MusC.
Range:
0.5 to 32ng/ml
Sample Type:
Serum
Sensitivity:
50pg/ml
Specificity:
Detects mouse and rat Nampt. Does not cross-react with human Nampt, mouse adiponectin, mouse resistin, mouse vaspin or mouse RBP4.
Transportation:
Non-hazardous
UNSPSC Category:
ELISA Kits
UNSPSC Number:
41116126
Use & Stability:
12 months after the day of manufacturing. See expiry date on ELISA Kit box.

References

Nampt secreted from cardiomyocytes promotes development of cardiac hypertrophy and adverse ventricular remodeling: V.B. Pillai, et al.; Am. J. Physiol. Heart Circ. Physiol. 304, H415 (2013) | Hepatectomy-related hypophosphatemia: a novel phosphaturic factor in the liver-kidney axis: K. Nomura, et al.; J. Am. Soc. Nephrol. 25, 761 (2014) | Nicotinamide phosphoribosyltransferase inhibitor is a novel therapeutic candidate in murine models of inflammatory lung injury: L. Moreno-Vinasco, et al.; Am. J. Respir. Cell Mol. Biol. 51, 223 (2014) | Hepatic NAD+ deficiency as a therapeutic target for non-alcoholic fatty liver disease in ageing: C.-C. Zhou, et al.; Br. J. Pharmacol. 173, 2352 (2016) | NAMPT inhibitor protects ischemic neuronal injury in rat brain via anti-neuroinflammation: C.-X. Chen, et al.; Neuroscience 356, 193 (2017) | Neutralization of extracellular NAMPT (nicotinamide phosphoribosyltransferase) ameliorates experimental murine colitis: G. Colombo, et al.; J. Mol. Med. 98, 595 (2020) | CD38 downregulation modulates NAD+ and NADP(H) levels in thermogenic adipose tissues: A. Benzi, et al.; BBA Mol. Cell Biol. Lipids 1866, 158819 (2021)