EpiFectagen

The delivery of foreign DNA into eukaryotic cells is one of the most common molecular biology techniques to study biological mechanisms. However, unlike transformed cell lines, the efficient transfection of primary cells can be a problem. EpiFectagen is a cationic polymer-based transfection system specifically designed and optimized for efficient transfection of primary epithelial cells. Transfection with EpiFectagen can be carried out in the presence of antibiotics and serum. Instead of normal two-day transfection, an optimized one-day transfection procedure can be performed for time-saving and highly reproducible transfection. 1.5 ml of EpiFectagen reagent is sufficient for up to 100 transfections per well in 96-well plate.

Upon receipt, aliquot and store EpiFectagen reagent A at -20°C, avoid repeated freezing/thawing cycles. Once thawed, store EpiFectagen reagent A at 4°C and use in a month. EpiFectagen reagent B can be kept at 4°C.