ProSci

IKK gamma Antibody

Product Code:
 
PSI-2335
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 μg/mL.
2 / 3
Immunocytochemistry of IKK gamma in HeLa cells with IKK gamma antibody at 5 μg/mL.
3 / 3
Immunofluorescence of IKK gamma in Hela cells with IKK gamma antibody at 20 μg/mL.<br><br>Red: IKK gamma Antibody (2335) <br> Blue: DAPI staining
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Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 μg/mL.
Immunocytochemistry of IKK gamma in HeLa cells with IKK gamma antibody at 5 μg/mL.
Immunofluorescence of IKK gamma in Hela cells with IKK gamma antibody at 20 μg/mL.<br><br>Red: IKK gamma Antibody (2335) <br> Blue: DAPI staining

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CodeSizePrice
PSI-2335-0.02mg0.02mg£150.00
Quantity:
PSI-2335-0.1mg0.1mg£449.00
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Further Information

Additional Names:
IKK gamma Antibody: IP, IP1, IP2, FIP3, IPD2, NEMO, FIP-3, Fip3p, AMCBX1, ZC2HC9, IKK-gamma, NF-kappa-B essential modulator
Application Note:
IKK gamma antibody can be used for detection of IKK gamma by Western blot at 1 μg/mL. A 52 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
IKK gamma Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The IκB kinase (IKKα and IKKβ) phosphorylates IkB and mediates NF-κB activation. A novel molecule in the IKK complex was recently identified and termed IKKγ/NEMO/FIP3/IKKAP1. IKKγ interacts with IKKβ and is required for the activation of IKK complex and NF-κB by LPS, PMA, TNF, and IL-1 stimulation. FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κB activation.
Background References:
  • Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-γ is an essential regulatory subunit of the IκB kinase complex. Nature 1998;395(6699):297-300
  • Yamaoka S, Courtois G, Bessia C, Whiteside ST, Weil R, Agou F, Kirk HE, Kay RJ, Israel A. Complementation cloning of NEMO, a component of the IκB kinase complex essential for NF-κB activation. Cell. 1998;93(7):1231-40.
  • Li Y, Kang J, Friedman J, Tarassishin L, Ye J, Kovalenko A, Wallach D, Horwitz MS. Identification of a cell protein (FIP-3) as a modulator of NF-κB activity and as a target of an adenovirus inhibitor of tumor necrosis factor α-induced apoptosis. Proc Natl Acad Sci U S A 1999;96(3):1042-7
  • Mercurio F, Murray BW, Shevchenko A, Bennett BL, Young DB, Li JW, Pascual G, Motiwala A, Zhu H, Mann M, Manning AM. IκB kinase (IKK)-associated protein 1, a common component of the heterogeneous IKK complex. Mol Cell Biol. 1999;19(2):1526-38.
Buffer:
IKK gamma Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
IKK gamma antibody was raised against a 17 amino acid peptide near the carboxy terminus of human IKK gamma.

The immunogen is located within the last 50 amino acids of IKK gamma.
NCBI Gene ID #:
8517
NCBI Official Name:
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
NCBI Official Symbol:
IKBKG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
AF074382
Protein GI Number:
3641279
Purification:
IKK gamma Antibody is DEAE purified.
Research Area:
Signal Transduction
SPECIFICITY:
IKK gamma has no cross response to IKK alpha or IKK beta.
Swissprot #:
Q9Y6K9
User NOte:
Optimal dilutions for each application to be determined by the researcher.