ProSci

DC-SIGN Antibody

Product Code:
 
PSI-2347
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot detection of DC-SIGN in human small intestine at (A) 1 and (B) 2 μg/ml.
2 / 3
Immunohistochemistry of DC-SIGN in human lymph node tissue with DC-SIGN antibody at 10 μg/mL.
3 / 3
Immunofluorescence of DC-SIGN in Human Lymph Node tissue with DC-SIGN antibody at 20 μg/mL.
❮❯

Western blot detection of DC-SIGN in human small intestine at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of DC-SIGN in human lymph node tissue with DC-SIGN antibody at 10 μg/mL.
Immunofluorescence of DC-SIGN in Human Lymph Node tissue with DC-SIGN antibody at 20 μg/mL.

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CodeSizePrice
PSI-2347-0.02mg0.02mg£150.00
Quantity:
PSI-2347-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
DC-SIGN Antibody: CDSIGN, CLEC4L, DC-SIGN, DC-SIGN1, CD209 antigen, C-type lectin domain family 4 member L
Application Note:
DC-SIGN antibody can be used for detection of DC-SIGN by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
DC-SIGN Antibody: Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes, where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN. ICAM-3 is the natural ligand for DC-SIGN. A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently. DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR. The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.
Background References:
  • Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell 2000;100:587-97.
  • Pohlmann S, Baribaud F, Lee B, et al. RW. DC-SIGN interactions with human immunodeficiency virus type 1 and 2 and simian immunodeficiency virus. J. Virol. 2001; 75:4664-72.
  • Geijtenbeek TB, Torensma R, van Vliet SJ, et al. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell 2000; 100:575-85.
  • Soilleux EJ, Barten R, and Trowsdale J. DC-SIGN; a related gene, DC-SIGNR; and CD23 form a cluster on 19p13. J. Immunol. 2000; 165:2937-42.
Buffer:
DC-SIGN Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
DC-SIGN antibody was raised against a synthetic peptide corresponding to 21 amino acids near the center of human DC-DIGN.

The immunogen is located within the last 50 amino acids of DC-SIGN.
NCBI Gene ID #:
30835
NCBI Official Name:
CD209 molecule
NCBI Official Symbol:
CD209
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 44 kDa

Observed: 47 kDa
Protein Accession #:
Q9NNX6
Protein GI Number:
46396012
Purification:
DC-SIGN Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease
Swissprot #:
Q9NNX6
User NOte:
Optimal dilutions for each application to be determined by the researcher.