IRAK-4 Antibody
PSI-3125
Rabbit
IgG
Polyclonal
RUO
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
No additional charges, what you see is what you pay! *
Code | Size | Price |
---|
PSI-3125-0.02mg | 0.02mg | £150.00 |
PSI-3125-0.1mg | 0.1mg | £449.00 |
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Further Information
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- Cao et al. Science 1996; 271:1128-31
- Muzio et al. Science 1997; 278:1612-5.
- Wesche et al. J. Biol. Chem. 1999; 274:19403-10.
- Li et al. Proc. Natl. Acad. Sci. USA 2002; 99:5567-72.
The immunogen is located within the last 50 amino acids of IRAK4.
Observed: 55 kD
Independent Antibody Validation in Cell lines (Figure 2) shows similar IRAK4 expression profile in human cell lines detected by two independent anti-IRAK4 antibodies that recognize different epitopes, 3125 against C-terminus domain and 24-025 against recombinant fusion protein. IRAK4 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.
KO validation (Figure 6): Anti-IRAK4 antibody (3125) specificity was further verified by IRAK4 knockout and mutant mice. IRAK4 signal in the macrophages of IRAK KO mice and mutant mice was disrupted as compared to wild type mice.
Regulated expression validation (Figure 7): IRAK4 expression detected by anit-IRAK4 antibodies (3125) was reduced with CSK-4 treatment, which was rescued by proteasome inhibitors.
KD validation (Figure 8): Anti-IRAK4 antibody (3125) specificity was further verified by IRAK4 knockdown. IRAK4 signal was disrupted in HEK293T cells transfected with IRAK4 siRNAs.
Documents
References
- Koziczak-Holbro et al. IRAK-4 kinase activity-dependent and -independent regulation of lipopolysaccharide-inducible genes. Eur J Immunol. 2008;38(3):788-96.PMID: 18266302
- Hatao et al. Prolonged Toll-like receptor stimulation leads to down-regulation of IRAK-4 protein. J Leukoc Biol. 2004;76(4):904-8. PMID: 15258191
- Heinz et al. The death domain-containing protein Unc5CL is a novel MyD88-independent activator of the pro-inflammatory IRAK signaling cascade. Cell Death Differ. 2012;19(4):722-31. PMID: 22158417
- Koziczak-Holbro et al. IRAK-4 kinase activity is required for interleukin-1 (IL-1)receptor- and toll-like receptor 7-mediated signaling and gene expression. J Biol Chem. 2007;282(18):13552-60. PMID: 17337443
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