cIAP Antibody
Product Code:
PSI-3325
PSI-3325
Host Type:
Rabbit
Rabbit
Antibody Isotype:
IgG
IgG
Antibody Clonality:
Polyclonal
Polyclonal
Regulatory Status:
RUO
RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
No additional charges, what you see is what you pay! *
Code | Size | Price |
---|
PSI-3325-0.02mg | 0.02mg | £150.00 |
Quantity:
PSI-3325-0.1mg | 0.1mg | £449.00 |
Quantity:
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Typical lead time: 14-21 working days.
Contact us for more accurate information.
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Further Information
Additional Names:
cIAP Antibody: API1, MIHB, HIAP2, RNF48, cIAP1, Hiap-2, c-IAP1, API1, IAP2, Baculoviral IAP repeat-containing protein 2, C-IAP1, IAP-2
Application Note:
WB: 1-4 μg/mL; IHC: 10 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
cIAP Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The two isoforms of c-IAP (c-IAP1 and c-IAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7. The c-IAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF-α by its receptor. Omi, a negative regulator of c-IAP, inhibits its activity by catalytically cleaving c-IAP. Another negative regulator, Smac/DIABLO, acts by enhancing the auto-ubiquitization activity of c-IAP.
Background References:
- Schimmer. Cancer Res. 2004; 64:7183-90.
- Rothe et al. Cell 1995; 83:1243-52.
- Deveraux et al. EMBO J. 1998; 17:2215-23.
- Li et al. Nature 2002; 416:345-7.
Buffer:
cIAP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (93%), Chicken: (92%)
Immunogen:
Anti-cIAP antibody (3325) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human cIAP. c-IAP antibody detects both c-IAP1 and c-IAP2.
The immunogen is located within amino acids 540-590 of cIAP.
The immunogen is located within amino acids 540-590 of cIAP.
ISOFORMS:
Human cIAP has 2 isoforms, including isoform 1 (618aa, 70kD) and isoform 2 (569aa, 64kD). Mouse cIAP has one isoform (612aa, 70kD) and Rat cIAP also has one isoform (589aa, 67kD). 3325 can detect human, mouse and rat.
NCBI Gene ID #:
329
NCBI Official Name:
baculoviral IAP repeat-containing 2
NCBI Official Symbol:
BIRC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 70kD
Observed: 70kD
Observed: 70kD
Protein Accession #:
NP_001157
Protein GI Number:
4502141
Purification:
cIAP Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Cancer
Swissprot #:
Q13490
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Independent Antibody Validation in Cell lines (Figure 2) shows similar cIAP expression profile in human, mouse and rat cell lines detected by two independent anti-cIAP antibodies that recognize different epitopes, 3325 against C-terminus domain and competitor antibody against recombinant protein. cIAP proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.
Documents
References
- Nuutinen et al. The effect of microenvironmental CD40 signals on TRAIL- and drug-induced apoptosis in follicular lymphoma cells. Scand J Immunol. 2009;70(6):565-73.PMID: 19906199
- Hahm and Singh. Withaferin A-induced apoptosis in human breast cancer cells is associated with suppression of inhibitor of apoptosis family protein expression. Cancer Lett. 2013;334(1):101-8. PMID: 22935676
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