Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
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United States.
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Further Information
IRF3 Antibody: Interferon regulatory factor 3, IRF-3
IRF3 antibody can be used for detection of IRF3 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
IRF3 Antibody: Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells. Following viral infection, IRF3 can be activated by IκB kinase-ε and TANK-binding kinase 1 (TBK1), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA. IRF3 exists as at least two distinct isoforms.
- Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54
- Au WC, Moore PA, Lowther W, et al. Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes. Proc. Natl. Acad. Sci. USA 1995; 92:11657-61.
- Fitzgerald KA, McWhirter SM, Faia KL, et al. IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway. Nat. Immunol. 2003; 4:491-6.
- Sharma S, Tenoever BR, Grandvaux N, et al. Triggering the interferon antiviral response through an IKK-related pathway. Science 2003; 300:1148-51.
IRF3 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine: (86%), Pig: (79%)
IRF3 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human IRF3.
The immunogen is located within amino acids 330 - 380 of IRF3.
3661
interferon regulatory factor 3
IRF3
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
43, 47, 50 kDa
NP_001562
4504725
IRF3 Antibody is affinity chromatography purified via peptide column.
Chemokines & Cytokines,Homeostasis
At least three isoforms of IRF3 are known to exist.
Q14653
Optimal dilutions for each application to be determined by the researcher.