ProSci

SARS Matrix Antibody

Product Code:
 
PSI-3529
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
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<strong>Figure 1 Western Blot Validation with SARS-CoV-2 Matrix Recombinant Protein</strong><br>Loading: SARS-CoV-2 Matrix recombinant protein. Antibodies: SARS-CoV-2/SARS-CoV Matrix (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 10 ng Matrix protein, Lane 2: 25 ng Matrix protein and Lane 3: 50 ng Matrix protein.
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<strong>Figure 2 ELISA Test </strong><br>Antibodies: SARS-CoV-2/SARS-CoV Matrix Antibody, 3529. A direct ELISA was performed using Matrix recombinant protein as coating antigen and the anti-SARS-CoV-2/SARS-CoV Matrix antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Detection range is from 8ng/mL to 2000ng/mL.
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<strong>Figure 3 ELISA Test </strong><br>Antibodies: SARS-CoV-2/SARS-CoV Matrix Antibody, 3529 (1 μg/mL). A direct ELISA was performed using immunogen (3529P) as coating antigen and the anti-SARS-CoV-2/SARS-CoV Matrix antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000ng/mL.
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<strong>Figure 1 Western Blot Validation with SARS-CoV-2 Matrix Recombinant Protein</strong><br>Loading: SARS-CoV-2 Matrix recombinant protein. Antibodies: SARS-CoV-2/SARS-CoV Matrix (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 10 ng Matrix protein, Lane 2: 25 ng Matrix protein and Lane 3: 50 ng Matrix protein.
<strong>Figure 2 ELISA Test </strong><br>Antibodies: SARS-CoV-2/SARS-CoV Matrix Antibody, 3529. A direct ELISA was performed using Matrix recombinant protein as coating antigen and the anti-SARS-CoV-2/SARS-CoV Matrix antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Detection range is from 8ng/mL to 2000ng/mL.
<strong>Figure 3 ELISA Test </strong><br>Antibodies: SARS-CoV-2/SARS-CoV Matrix Antibody, 3529 (1 μg/mL). A direct ELISA was performed using immunogen (3529P) as coating antigen and the anti-SARS-CoV-2/SARS-CoV Matrix antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000ng/mL.

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CodeSizePrice
PSI-3529-0.02mg0.02mg£150.00
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PSI-3529-0.1mg0.1mg£449.00
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Further Information

Additional Names:
SARS-CoV-2/SARS-CoV Matrix Antibody: SARS-CoV-2 Matrix protein, SARS Matrix protein, Membrane protein, E1 glycoprotein, M protein
Application Note:
WB: 1 μg/mL.

Antibody validated: SARS-CoV-2/SARS-CoV Matrix antibody can be used for the detection of SARS-CoV-2/SARS-CoV Matrix protein in ELISA. It will detect 5 ng of free peptide at 1 μg/mL.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019?20 coronavirus outbreak (1). SARS-CoV-2 is the seventh member of the enveloped, positive-stranded RNA viruses that are able to infect humans. The SARS-CoV-2 genome, like other coronaviruses, encodes for multiple structural and nonstructural proteins. The structural proteins include spike protein (S), envelope protein (E), membrane glycoprotein (M), nucleocapsid phosphoprotein (N), and the nonstructural proteins include open reading frame 1ab (ORF1ab), ORF3a, ORF6, ORF7a, ORF8, and ORF10 (2).The membrane (M) protein or matrix protein is the most abundant structural protein and defines the shape of the viral envelope (3). It is an integral membrane protein involved in the budding of the viral particles and interacts with S (Spike) protein. It involves in organization of the nucleoprotein inside, which includes many copies of the N (nucleocapsid) protein bound to the genomic RNA. The M protein holds dominant cellular immunogenicity and has been determined as a protective antigen in humoral responses, which suggests it would serve as a potential target in vaccine design (4).
Background References:
  • Zhu et al. N Engl J Med.?2020 Feb 20;382(8):727-733.
  • Kiyotani et al. J Hum Genet.?2020 Jul;65(7):569-575.
  • Neuman et al. J Struct Biol. 2011;174(1):11?22.
  • Liu et al. J Infect Dis. 2010;202(8):1171-80.
Buffer:
SARS-CoV-2/SARS-CoV Matrix Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV-2 Matrix protein: (identity 80%, homology 93%)
Immunogen:
Anti-SARS-CoV-2/SARS-CoV Matrix antibody (3529) was raised against a peptide corresponding to 15 amino acids near the carboxy-terminus of SARS-CoV Matrix protein.

The immunogen is located within the last 50 amino acids of SARS-CoV Matrix.
ISOFORMS:
SARS-CoV Matrix has one isoform (222aa).
NCBI Gene ID #:
43740571
NCBI Official Name:
Membrane protein
NCBI Official Symbol:
M
NCBI Organism:
SARS-CoV-2
Physical State:
Liquid
Protein Accession #:
QHO62114.1
Protein GI Number:
1800455127
Purification:
SARS-CoV-2/SARS-CoV Matrix Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease,COVID-19
Swissprot #:
QHO62114.1
User NOte:
Optimal dilutions for each application to be determined by the researcher.

References

  1. Jaume, et al. Anti-severe acute respiratory syndrome coronavirus spike antibodies trigger infection of human immune cells via a pH- and cysteine protease-independent Fc?R pathway. J Virol. 2011 Oct;85(20):10582-97. doi: 10.1128/JVI.00671-11. Epub 2011 Jul 20.PMID: 21775467