ProSci

SARS Envelope Antibody

Product Code:
 
PSI-3533
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
<strong>Figure 1 ELISA Test </strong><br> Antibodies: SARS-CoV Envelope Antibody, 3533 (1 μg/mL). A direct ELISA was performed using immunogen as coating antigen and the anti-SARS-CoV Envelope antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000 ng/mL.

Figure 1 ELISA Test
Antibodies: SARS-CoV Envelope Antibody, 3533 (1 μg/mL). A direct ELISA was performed using immunogen as coating antigen and the anti-SARS-CoV Envelope antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000 ng/mL. 

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CodeSizePrice
PSI-3533-0.02mg0.02mg£150.00
Quantity:
PSI-3533-0.1mg0.1mg£453.00
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Further Information

Additional Names:
SARS-CoV Envelope Antibody: sM, 4, Envelope small membrane protein, E protein
Application Note:
SARS-CoV Envelope antibody can be used for the detection of SARS-CoV Envelope protein in ELISA. It will detect 5 ng of free peptide at 1 μg/mL.
Background:
SARS Envelope Antibody: A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2. Envelope protein is a small polypeptide that contains at least one ?-helical transmembrane domain. It involves in several aspects of the virus's life cycle, such as assembly, budding, envelope formation, and pathogenesis. E protein has membrane permeabilizing activity, which provides a possible rationale to inhibit in vitro ion channel activity of some synthetic coronavirus E proteins, and also viral replication.
Background References:
  • Marra et al. Science 2003;300:1399-404.
  • Rota et al. Science 2003;300:1394-9.
  • Navas-Nartin et al. J Neurovirol. 2004;10:75-85.
  • Pervushin et al. PLoS Pathog. 2009; 5(7): e1000511.
Buffer:
SARS-CoV Envelope Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV-2 Envelope protein: (identity 82%, homology 91%)
Immunogen:
Anti-SARS-CoV Envelope antibody (3533) was raised against a peptide corresponding to 11 amino acids near the carboxy terminus of SARS-CoV Envelope protein.

The immunogen is located within the last 50 amino acids of SARS-CoV Envelope.
ISOFORMS:
SARS-CoV Envelope has one isoform (75aa).
NCBI Gene ID #:
1489671
NCBI Official Name:
Envelope small membrane protein
NCBI Official Symbol:
E
NCBI Organism:
SARS coronavirus
Physical State:
Liquid
Protein Accession #:
P59637
Protein GI Number:
30173401
Purification:
SARS-CoV Envelope Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience,Infectious Disease,COVID-19
Swissprot #:
P59637
User NOte:
Optimal dilutions for each application to be determined by the researcher.

References

  1. Siu, et al. The M, E, and N structural proteins of the severe acute respiratory syndrome coronavirus are required for efficient assembly, trafficking, and release of virus-like particles. J Virol. 2008 Nov;82(22):11318-30. doi: 10.1128/JVI.01052-08. Epub 2008 Aug 27.PMID: 18753196