Further Information
BANF1 Antibody: BAF, NGPS, BCRP1, D14S1460, BAF, BCRG1, Barrier-to-autointegration factor, Breakpoint cluster region protein 1
WB: 0.5-2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
BANF1 Antibody: Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BANF1 also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BANF1 will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BANF1 is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA.
- Furukawa. J. Cell Sci. 1999; 112:2485-92.
- Cai et al. EMBO J. 2001; 20:4399-407.
- Chen and Engelman. Proc. Natl. Acad. Sci. USA 1998; 95:15270-4.
BANF1 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Mouse: (100%), Rat: (100%)
Anti-BANF1 antibody (4017) was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human BANF1.
The immunogen is located within the last 50 amino acids of BANF1.
Human BANF1 has one isoform (89aa, 10kD). Mouse BANF1 has one isoform (89aa, 10kD) and Rat BANF1 also has one isoform (89aa, 10kD). 4017 can detect human, mouse and rat.
8815
barrier to autointegration factor 1
BANF1
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 10kD
Observed: 12 kD
Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE.
AAH05942
13543577
BANF1 Antibody is affinity chromatography purified via peptide column.
Infectious Disease
O75531
Optimal dilutions for each application to be determined by the researcher.
Independent Antibody Validation in Cell lines (Figure 1) shows similar BANF1 expression profile in human and mouse cell lines detected by two independent anti-BANF1 antibodies that recognize different epitopes, 4017 against C-terminus domain and 4019 against N-terminus domain. BANF1 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.