ProSci

CRTH2 Antibody

Product Code:
 
PSI-4029
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of CRTH2 in human heart tissue lysate with CRTH2 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunohistochemistry of CRTH2 in human heart tissue with CRTH2 antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of CRTH2 in Human Heart cells with CRTH2 antibody at 20 μg/mL.
❮❯

Western blot analysis of CRTH2 in human heart tissue lysate with CRTH2 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of CRTH2 in human heart tissue with CRTH2 antibody at 2.5 μg/mL.
Immunofluorescence of CRTH2 in Human Heart cells with CRTH2 antibody at 20 μg/mL.

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CodeSizePrice
PSI-4029-0.02mg0.02mg£150.00
Quantity:
PSI-4029-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
CRTH2 Antibody: DP2, DL1R, CD294, CRTH2, GPR44, Prostaglandin D2 receptor 2, Chemoattractant receptor-homologous molecule expressed on Th2 cells
Application Note:
CRTH2 antibody can be used for detection of CRTH2 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
CRTH2 Antibody: The chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) is a recently identified receptor for the prostaglandin D2 (PGD2) in addition to the classic prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo. At least two different isoforms of CRTH2 are known to exist.
Background References:
  • Nagata K, Hirai K, Tanaka K, et al. CRTH2, an orphan receptor of T-helper-2-cells, is expressed on basophils and eosinophils and responds to mast cell-derived factor(s). FEBS Lett. 1999; 459:195-9.
  • Shichijo M, Sugimoto H, Nagao K, et al. Chemoattractant receptor-homologous molecule expressed on Th2 cells activation in vivo increases blood leukocyte counts and its blockage abrogates 13,14-dihydro-15-keto-prostaglandin D2-induced eosinophilia in rats. J. Pharmacol. Exp. Ther. 2003; 307:518-25.
  • Chevalier E, Stock J, Fisher T, et al. Cutting edge: chemoattractant receptor-homologous molecule expressed on Th2 cells plays a restricting role on IL-5 production and eosinophil recruitment. J. Immunol. 2005; 2056-60.

    (07-01D)
Buffer:
CRTH2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CRTH2 antibody was raised against a 15 amino acid synthetic peptide from near the center of human CRTH2.

The immunogen is located within amino acids 220 - 270 of CRTH2.
NCBI Gene ID #:
11251
NCBI Official Name:
prostaglandin D2 receptor 2
NCBI Official Symbol:
PTGDR2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004769
Protein GI Number:
153791424
Purification:
CRTH2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
Swissprot #:
Q9Y5Y4
User NOte:
Optimal dilutions for each application to be determined by the researcher.