ProSci

Emerin Antibody

Product Code:
 
PSI-4031
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of Emerin in human skeletal muscle tissue lysate with Emerin antibody at (A) 0.5 and (B) 1 μg/mL.
2 / 3
Immunohistochemistry of emerin in human skeletal muscle tissue with emerin antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of Emerin in Human Skeletal Muscle cells with Emerin antibody at 5 μg/mL.
❮❯

Western blot analysis of Emerin in human skeletal muscle tissue lysate with Emerin antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of emerin in human skeletal muscle tissue with emerin antibody at 2.5 μg/mL.
Immunofluorescence of Emerin in Human Skeletal Muscle cells with Emerin antibody at 5 μg/mL.

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CodeSizePrice
PSI-4031-0.02mg0.02mg£150.00
Quantity:
PSI-4031-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
Emerin Antibody: STA, EDMD, LEMD5, STA, Emerin
Application Note:
Emerin antibody can be used for detection of Emerin by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Emerin Antibody: Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family that includes proteins such as LAP2 and MAN1. Each family member, including Emerin, has an ~40 amino acid LEM-domains that binds barrier-to-autointegration (BANF1), a conserved chromatin protein that also serves as a host cell component of retroviral integration complexes, including that of HIV. Emerin is anchored at the inner membrane of the nuclear envelope where it binds to nuclear intermediate filaments that are formed by lamin proteins. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.
Background References:
  • Schirmer EC, Florens L, Guan T, et al. Nuclear membrane proteins with potential disease links found by subtractive proteomics. Science 2003; 301:1380-2.
  • Cai M, Huang Y, Ghirlando R, et al. Solution structure of the constant region of nuclear envelope protein LAP2 reveals two LEM-domain structures: one binds BAF and the other binds DNA. EMBO J. 2001; 20:4399-407.
  • Chen H and Engelman A. The barrier-to-autointegration protein is a host factor for HIV type 1 integration. Proc. Natl. Acad. Sci. USA 1998; 95:15270-4.
  • Hutchison CJ. Lamins: building blocks or regulators of gene expression Nat. Rev. Mol. Cell Biol. 2002; 3:848-58.
Buffer:
Emerin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Emerin antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Emerin.

The immunogen is located within the first 50 amino acids of Emerin.
NCBI Gene ID #:
2010
NCBI Official Name:
emerin
NCBI Official Symbol:
EMD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000108
Protein GI Number:
4557553
Purification:
Emerin Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease
Swissprot #:
P50402
User NOte:
Optimal dilutions for each application to be determined by the researcher.