Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
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Further Information
TEM5 Antibody: TEM5, KIAA1531, TEM5, G-protein coupled receptor 124, Tumor endothelial marker 5
TEM5 antibody can be used for detection of TEM5 by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
TEM5 Antibody: Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogensis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. These include TEMs such as TEM1, TEM5, TEM7 and TEM8. TEM5 is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. TEM5 is a seven-pass transmembrane receptor, unlike TEM1, TEM7 and TEM8 which span the membrane once. TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.
- Carson-Walter EB, Watkins DN, Nanda A, et al. Cell surface tumor endothelial markers are conserved in mice and humans. Cancer Res.2001; 61:6649-55.
- Yamamoto Y, Irie K, Nanda A, et al. Direct binding of the human homologue of the Drosophila disc large tumor suppressor gene to seven-pass transmembrane proteins, tumor endothelial marker 5 (TEM5), and a novel TEM5-like protein. Oncogene2004; 23:3889-97.
- Davies G, Cunnick GH, Mansel RE, et al. Levels of expression of endothelial markers specific to tumour-associated endothelial cells and their correlation with prognosis in patients with breast cancer. Clinical & Experimental Metastasis2004; 21:31-7.
- Vallon M and Essler M. Proteolytically processed soluble tumor endothelial marker (TEM) 5 mediates endothelial cell survival during angiogenesis by linking integrin alpha(v)beta3 to glycosaminoglycans. J. Biol. Chem.2006; 281:34179-88.
TEM5 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Mouse: (85%)
TEM5 antibody was raised against a 14 amino acid synthetic peptide near the center of human TEM5.
The immunogen is located within amino acids 560 - 610 of TEM5.
25960
G protein-coupled receptor 124
GPR124
Homo sapiens
Liquid
Q96PE1
221222450
TEM5 Antibody is affinity chromatography purified via peptide column.
Homeostasis
Q96PE1
Optimal dilutions for each application to be determined by the researcher.