Further Information
Rim2 Antibody: OBOE, RIM2, RAB3IP3, KIAA0751, Regulating synaptic membrane exocytosis protein 2, Rab-3-interacting molecule 2, RIM 2
Rim2 antibody can be used for detection of Rim2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Rim2 Antibody: Rab3-interacting molecules (RIMs) are synaptic proteins necessary for neuronal transmission and plasticity. Rim1 and Rim2 proteins are expressed in overlapping but distinct patterns throughout the brain. While the ablation of either gene was not lethal in mice, the deletion of both resulted in postnatal mortality. This lethality is due to a defect in neurotransmitter release; synapses without RIM proteins can still release neurotransmitters but are unable to do so in response to normal Ca2+ triggers. Like Rim1, Rim 2 is thought to be an effector protein for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner.
- Wang Y, Sugita S, and Sudhof TC. The RIM/NIM family of neuronal C2 domain proteins: interactions with Rab3 and a new class of Src homology 3 domain proteins. J. Biol. Chem. 2000; 275:20033-44.
- Liang F, Zhang B, Tang J, et al. RIM3gamma is a postsynaptic protein in the rat central nervous system. J. Comp. Neurol. 2007; 503:501-10.
- Shoch S, Mittelstaedt T, Kaeser PS, et al. Redundant functions of RIM1a and RIM2a in Ca2+-triggered neurotransmitter release. EMBO J. 2006; 25:5852-63.
Rim2 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Rim2 antibody was raised against a 17 amino acid synthetic peptide near the center of the human Rim2.
The immunogen is located within amino acids 250 - 300 of Rim2.
9699
regulating synaptic membrane exocytosis 2
RIMS2
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 39, 128, 148, 170 kDa
Observed: 128 kDa
NP_001093587
154354983
Rim2 Antibody is affinity chromatography purified via peptide column.
Neuroscience
Multiple isoforms of RIM2 are known to exist. This antibody is predicted to have no cross-reactivity to other Rim proteins.
Q9UQ26
Optimal dilutions for each application to be determined by the researcher.