Further Information
TNFAIP3 Antibody: A20, Tnfip3, Tumor necrosis factor alpha-induced protein 3, Putative DNA-binding protein A20, TNF alpha-induced protein 3
TNFAIP3 antibody can be used for detection of TNFAIP3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
TNFAIP3 Antibody: TNFAIP3, also known as A20, is located in chromosome band 6q23, a region that is often deleted in B cell lymphomas. Recently, it was identified as a tumor suppressor gene in Hodgkin lymphoma and several subtypes of non-Hodgkin lymphomas. TNFAIP3 was initially identified as a zinc-finger protein that is rapidly and transiently induced by TNF-α, inhibiting NF-κB-dependent gene expression, and protecting cells from TNF-α-cytotoxicity. Overexpression of TNFAIP3 also inhibits the TLR2- and TLR4-mediated interleukin-8 synthesis in airway epithelial cells, suggesting that TNFAIP3 also acts as a negative regulator of TLR-mediated inflammatory responses, thereby protecting the host against harmful over-responses to pathogens. At least two isoforms of TNFAIP3 are known to exist.
- Zhang YP, Matthiesen S, Harder R, et al. A 3-cM commonly deleted region in 6q21 in leukemias and lymphomas delineated by fluorescence in situ hybridization. Genes Chromosomes Cancer2000; 27:52-58.
- Schmitz R, Hansmann M-L, Bohle V, et al. TNFAIP3 (A20) is a tumor suppressor gene in Hodgkin lymphoma and primary mediastinal B cell lymphoma. J. Exp. Med.2009; 206:981-9.
- Honma K, Tsuzuki S, Nakagawa M, et al. TNFAIP3/A20 functions as a novel tumor suppressor gene in several subtypes of non-Hodgkins lymphomas. Blood2009; epub.
- Opipari AW Jr, Hu MN, Yabkowitz R, et al. The A20 zinc finger protein protects cells from tumor necrosis factor cytotoxicity. J. Biol. Chem.1992; 267:12424-7.
TNFAIP3 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
TNFAIP3 antibody was raised against a 17 amino acid synthetic peptide near the center of human TNFAIP3.
The immunogen is located within amino acids 340 - 390 of TNFAIP3.
21929
tumor necrosis factor, alpha-induced protein 3
Tnfaip3
Mus musculus
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 87 kDa
Observed: 95 kDa
Q60769
2498097
TNFAIP3 Antibody is affinity chromatography purified via peptide column.
Innate Immunity
Q60769
Optimal dilutions for each application to be determined by the researcher.