ProSci

PION Antibody

Product Code:
 
PSI-6161
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 5
Western blot analysis of PION in EL4 cell lysate with PION antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 5
Immunohistochemistry of PION in human brain tissue with PION antibody at 5 μg/mL.
3 / 5
Immunofluorescence of PION in Human Brain cells with PION antibody at 20 μg/mL.
4 / 5
Immunohistochemistry of PION in mouse brain tissue with PION antibody at 5 μg/mL.
5 / 5
Immunofluorescence of PION in mouse brain tissue with PION antibody at 20 μg/mL.<br><br>Red: PION Antibody (6161) <br> Blue: DAPI staining
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Western blot analysis of PION in EL4 cell lysate with PION antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of PION in human brain tissue with PION antibody at 5 μg/mL.
Immunofluorescence of PION in Human Brain cells with PION antibody at 20 μg/mL.
Immunohistochemistry of PION in mouse brain tissue with PION antibody at 5 μg/mL.
Immunofluorescence of PION in mouse brain tissue with PION antibody at 20 μg/mL.<br><br>Red: PION Antibody (6161) <br> Blue: DAPI staining

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CodeSizePrice
PSI-6161-0.02mg0.02mg£150.00
Quantity:
PSI-6161-0.1mg0.1mg£449.00
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Further Information

Additional Names:
PION Antibody: PION, PION, Gamma-secretase-activating protein, Protein pigeon homolog, GSAP
Application Note:
PION antibody can be used for detection of PION by Western blot at 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Background:
PION Antibody: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by one of two beta-secretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99 -amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the Alzheimer's disease patient's brains. PION, or GSAP, selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and the APP C-terminal fragment, suggesting that PION may be a potential therapeutic target for the treatment of Alzheimer's disease.
Background References:
  • Ponte P, Gonzalez-DeWhitt P, Schilling J, et al. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature1988; 331:525-77.
  • Selkoe DJ. Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease. Annu. Rev. Cell Biol.1994; 10:373-403.
  • He G, Luo W, Li P, et al. Gamma-secretase activating protein is a therapeutic target for Alzheimer's diease. Nature2010; 467:95-9.
Buffer:
PION Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PION antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PION.

The immunogen is located within amino acids 770 - 820 of PION.
NCBI Gene ID #:
54103
NCBI Official Name:
pigeon homolog (Drosophila)
NCBI Official Symbol:
PION
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_059135
Protein GI Number:
148612805
Purification:
PION Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
SPECIFICITY:
Multiple isoforms of PION are known to exist. PION antibody is predicted to not cross-react with other F-box protein family members.
Swissprot #:
A4D1B5
User NOte:
Optimal dilutions for each application to be determined by the researcher.